Research Articles
edited by Twyman Research Management
Abdelhafiz I, Gerth S, Claussen J, Weule M, Hufnagel E, Vilcinskas A, Lee KZ (2024) Radioactivity and GMO-free sterile insect technology for the sustainable control of the invasive pest Drosophila suzukii. Adv Biol 26, e2400100. DOI
An X, Gu Q, Wang J, Chang T, Zhang W, Wang JJ, Niu J (2024) Insect-specific RNA virus affects the stylet penetration activity of brown citrus aphid (Aphis citricidus) to facilitate its transmission. Insect Sci 31 (1) 255–270. DOI
Bayon JL, Shih C, Craig SL, Steinmetz NF (2024) A simple swell-and-click method for the covalent attachment of virus-like particles to polymer hydrogels. Mat Today Chem 38, 102100. DOI
Boll EJ, Lopez DV, Terne M, Hessing S, Parschat K, Jensen SR (2024) Human milk oligosaccharides differentially support gut barrier integrity and enhance Th1 and Th17 cell effector responses in vitro. Front Immunol 15, 1359499. DOI
Cheah BH, Chen YL, Zou XY, Lee KT, Shih YH, Lin YF (2024) Bacillus subtilis var. natto promotes tobacco plant growth under normal conditions and in the presence of sodium bicarbonate. Biolog Plant 68, 52–59. DOI
Erkoc P, Schiffmann S, Ulshöfer T, Henke M, Marner M, Krämer J, Predel R, Schäberle TF, Hurka S, Dersch L, Vilcinskas A, Fürst R, Lüddecke T (2024) Determining the pharmacological potential and biological role of linear pseudoscorpion toxins via functional profiling. iScience (online first 8/6/2024). DOI
Hellmann MJ, Moerschbacher BM, Cord-Landwehr S (2024) Fast insights into chitosan-cleaving enzymes by simultaneous analysis of polymers and oligomers through size exclusion chromatography. Sci Rep 14 (1) 3417. DOI
Johnson KA, Budicini MR, Bhattarai N, Sharma T, Urata S, Gerstman BS, Chapagain PP, Li S, Stahelin RV (2024) PI(4,5)P2 binding sites in the Ebola virus matrix protein VP40 modulate assembly and budding. J Lipid Res 65 (3) 100512. DOI
Kaczor M, Malicki S, Folkert J, Dobosz E, Bryzek D, Chruscicka-Smaga B, Greczan M, Wesół-Kucharska D, Piątosa B, Samborowska E, Madzio J, Książyk J, Ehmke Vel Emczyńska E, Hajdacka M, Potempa J, Młynarski W, Rokicki D, Veillard F (2024) Neutrophil functions in patients with neutropenia due to glycogen storage disease type 1b treated with empagliflozin. Blood Adv 8 (11) 2790–2802. DOI
Mäckelmann S, Känel A, Kösters LM, Lyko P, Prüfer D, Noll GA, Wicke S (2024) Gene complementation analysis indicates that parasitic dodder plants do not depend on the host FT protein for flowering. Plant Commun 5 (5) 100826. DOI
Mizgalska D, Malicki S, Golda A, Chruścicka-Smaga B, Potempa J (2024) Screening and characterization of aptamers recognizing the periodontal pathogen Tannerella forsythia. FEBS Open Bio 14 (3) 498–504. DOI
Mücke Y, Jablonka N, Rimann N, Grisch-Chan HM, Hoffmann B, Schillberg S, Thöny B, Rasche S (2024) A phenylalanine-free recombinant nutritional protein for the dietary management of phenylketonuria. J Inherit Metab Dis (online first 23/2/2024). DOI
Nausch H, Baldan M, Teichert K, Lutz J, Claussen C, Bortz M, Buyel JF (2024) Simulation and optimization of nutrient uptake and biomass formation using a multi-parameter Monod-type model of tobacco BY-2 cell suspension cultures in a stirred-tank bioreactor. Front Plant Sci 14, 1183254. DOI
Omokungbe B, Centurión A, Stiehler S, Morr A, Vilcinskas A, Steinbrink A, Hardes K (2024) Gene silencing in the aedine cell lines C6/36 and U4.4 using long double-stranded RNA. Parasit Vectors 17 (1) 255. DOI
Opdensteinen P, Knödler M, Buyel JF (2024) Production of enzymes for the removal of odorous substances in plant biomass. Prot Exp Purif 214, 106379. DOI
Rinklef A, Behrmann SC, Löffler D, Erner J, Meyer MV, Lang C, Vilcinskas A, Lee KZ (2024) Prevalence in potato of 'Candidatus Arsenophonus Phytopathogenicus' and 'Candidatus Phytoplasma Solani' and their transmission via adult Pentastiridius leporinus. Insects 15 (4) 275. DOI
Roelfs KU, Känel A, Twyman RM, Prüfer D, Schulze Gronover C (2024) Epigenetic variation in early and late flowering plants of the rubber-producing Russian dandelion Taraxacum koksaghyz provides insights into the regulation of flowering time. Sci Rep 14, 4283. DOI
Schäufele TJ, Kolbinger A, Friedel J, Gurke R, Geisslinger G, Weigert A, Pierre S, Scholich K (2024) Meloxicam treatment disrupts the regional structure of innate inflammation sites by targeting the pro-inflammatory effects of prostanoids. Br J Pharmacol 181 (7) 1051–1067. DOI
Schmidt FJ, Grundmann L, Lahme M, Seidemann M, Schwarze A, Lichtenauer S, Twyman RM, Prüfer D, Noll GA (2024) COL2-dependent photoperiodic floral induction in Nicotiana sylvestris seems to be lost in the N. sylvestris × N. tomentosiformis hybrid N. tabacum. Front Plant Sci 14, 1249879. DOI
Serra Mari R, Schrinner S, Finkers R, Ziegler FMR, Arens P, Schmidt MH, Usadel B, Klau GW, Marschall T (2024) Haplotype-resolved assembly of a tetraploid potato genome using long reads and low-depth offspring data. Genome Biol 25 (1) 26. DOI
Sobrino-Mengual G, Alvarez D, Twyman RM, Gerrish C, Fraser PD, Capell T, Christou P (2024) Activation of the native PHYTOENE SYNTHASE 1 promoter by modifying near-miss cis-acting elements induces carotenoid biosynthesis in embryogenic rice callus. Plant Cell Rep 43 (5) 118. DOI
Sobrino-Mengual G, Armario-Nájera V, Balieu J, Walet-Balieu ML, Saba-Mayoral A, Pelacho AM, Capell T, Christou P, Bardor M, Lerouge P (2024) The SARS-CoV-2 spike protein receptor-binding domain expressed in rice callus features a homogeneous mix of complex-type glycans. Int J Mol Sci 25 (8) 4466. DOI
Tschongov T, Konwar S, Busch A, Sievert C, Hartmann A, Noris M, Gastoldi S, Aiello S, Schaaf A, Panse J, Zipfel PF, Dabrowska-Schlepp P, Häffner K (2024) Moss-produced human complement factor H with modified glycans has an extended half-life and improved biological activity. Front Immunol 15, 1383123. DOI
Wang ZG, Qin CY, Chen Y, Yu XY, Chen RY, Niu J, Wang JJ (2024) Fusion dsRNA designs incorporating multiple target sequences can enhance the aphid control capacity of an RNAi-based strategy. Pest Manag Sci 80 (6) 2689–2697. DOI
Wang ZW, Zhao J, Li GY, Hu D, Wang ZG, Ye C, Wang JJ (2024) The endosymbiont Serratia symbiotica improves aphid fitness by disrupting the predation strategy of ladybeetle larvae. Insect Sci (online first 9/1/2024). DOI
Windfelder AG, Müller FHH, McLarney B, Hentschel M, Böhringer AC, von Bredow CR, Leinberger FH, Kampschulte M, Maier L, von Bredow YM, FlockeV, Merzendorfer H, Krombach GA, Vilcinskas A, Grimm J, Trenczek TE, Flögel U (2024) High-throughput screening of caterpillars as alternative platform for modeling of gut inflammation. Dev Comp Immunol 148, 105026. DOI
Windfelder AG, Steinbart J, Scherberich J, Krombach GA, Vilcinskas A (2024) An enteric ultrastructural surface atlas of the model insect Manduca sexta. iScience 27 (4) 109410. DOI
Preprints
Dersch L, Stahlhut A, Eichberg J, Paas A, Hardes K, Vilcinskas A, Lüddecke T (2024) Engineering a wolf spider A-family toxin towards increased antimicrobial activity but low toxicity. bioRxiv 2024.03.04.583312. DOI
Dresler J, Herzig V, Vilcinskas A, Lüddecke T (2024) Enlighting the toxinological dark matter of spider venom enzymes. bioRxiv 2024.02.27.582330. DOI
Rocchetti L, Rodriguez M, Pieri A, Papalini S, De Antoni L, Vitali E, Vincze A, Brezeanu C, Bellucci E, Nanni L, Rossato M, Delledonne M, Bitocchi E, Papa R (2024) Landscape genomics highlights the adaptive evolution of chickpea. bioRxiv 2024.06.06.597750. DOI
Schulte L, Uhrig L, Eichberg J, Schwartze M, Auth I, Schulz M, Lindner T, Hien P, Hardes K, Vilcinskas A, Lüddecke T (2024)Comparative venom analysis between melanistic and normally-colored phenotypes of the common adder (Vipera berus). bioRxiv 2024.03.18.585504. DOI
Susek K, Franco E, Tomaszewska M, Kroc M, Jamil H, Tanwar U, Nelson MN, Papa R, Delledonne M, Jackson SA (2024) The unexplored diversity of wild lupins provides rich genomic resources and insights into lupin evolution. bioRxiv 2024.03.07.583883. DOI
Affonso de Oliveira JF, Zhao Z, Xiang Y, Shin MD, Villaseñor KE, Deng X, Shukla S, Chen S, Steinmetz NF (2023) COVID-19 vaccines based on viral nanoparticles displaying a conserved B-cell epitope show potent immunogenicity and a long lasting antibody response. Front Microbiol 14, 1117494. DOI
Albuquerque W, Ghezellou P, Lee KZ, Schneider Q, Gross P, Kessel T, Omokungbe B, Spengler B, Vilcinskas A, Zorn H, Gand M (2023) Peptidomics as a tool to assess the cleavage of wine haze proteins by peptidases from Drosophila suzukii larvae. Biomolecules 13 (3) 451. DOI
Bhattacharya A, Mukherjee R, Kuncha SK, Brunstein M, Rathore R, Junek S, Münch C, Dikic I (2023) A lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins. Nature Cell Biol 25, 685–698. DOI
Behrmann SC, Rinklef A, Lang C, Vilcinskas A, Lee KZ (2023) Potato (Solanum tuberosum) as a new host for Pentastiridius leporinus (Hemiptera: Cixiidae) and Candidatus Arsenophonus phytopathogenicus. Insects 14 (3) 281. DOI
Bellucci E, Benazzo A, Xu C, Bitocchi E, Rodriguez M, Alseekh S, Di Vittori V, Gioia T, Neumann K, Cortinovis G, Frascarelli G, Murube E, Trucchi E, Nanni L, Ariani A, Logozzo G, Shin JH, Liu C, Jiang L, Ferreira JJ, Campa A, Attene G, Morrell PL, Bertorelle G, Graner A, Gepts P, Fernie AR, Jackson SA, Papa R (2023) Selection and adaptive introgression guided the complex evolutionary history of the European common bean. Nature Commun 14 (1) 1908. DOI
Bernau CR, Buyel JF (2023) The use of antifoam agents to eliminate bubbles during biotechnological sample analysis. Disc Cov Eng 3, 4. DOI
Chen YJ, Cheah BH, Lin CY, Ku YT, Kuo CH, Zhang YY, Chen BR, Olga NTN, Hsieh CH, Yeh PM, Yeo FKS, Lin YP, Chuang WP, Lee CR, Ting HM (2023) Inducible chemical defenses a in wild mung bean accessions confer resistance to Spodoptera litura at the expense of with drought tolerance. Env Exp Bot 205, 105100. DOI
Fiebig A, Leibl V, Oostendorf D, Lukaschek S, Frömbgen J, Masoudi M, Kremer AE, Strupf M, Reeh P, Düll M, Namer B (2023) Peripheral signaling pathways contributing to non-histaminergic itch in humans. J Transl Med 21 (1) 908. DOI
Fischer ML, Yepes Vivas SA, Wielsch N, Kirsch R, Vilcinskas A, Vogel H (2023) You are what you eat – ecological niche and microhabitat influence venom activity and composition in aquatic bugs. Proc Biol Sci 290 (1995) 20222064. DOI
González A, Covarrubias-Pinto A, Bhaskara RM, Glogger M, Kuncha SK, Xavier A, Seemann E, Misra M, Hoffmann ME, Bräuning B, Balakrishnan A, Qualmann B, Dötsch V, Schulman BA, Kessels MM, Hübner CA, Heilemann M, Hummer G, Dikić I (2023) Ubiquitination regulates ER-phagy and remodelling of endoplasmic reticulum. Nature 618 (7964) 394–401. DOI
Guzman J, Won M, Poehlein A, Sombolestani AS, Mayorga-Ch D, Laureys D, Clippeleer J, Kämpfer P, Daniel R, Vilcinskas A, Vandamme P, Kwon SW (2023) Aristophania vespae gen. nov., sp. nov., isolated from wasps, is related to Bombella and Oecophyllibacter, isolated from bees and ants. Int J Syst Evol Microbiol 73 (2) 005699. DOI
Holst AQ, Myers P, Rodríguez-García P, Hermes GDA, Melsaether C, Baker A, Jensen SR, Parschat K (2023) Infant formula supplemented with five human milk oligosaccharides shifts the fecal microbiome of formula-fed infants closer to that of breastfed infants. Nutrients 15 (14) 3087. DOI
Jung E, Chung YH, Mao C, Fiering SN, Steinmetz NF (2023) The potency of cowpea mosaic virus-based particles for in situ vaccination is unaffected by the specific encapsidated viral RNA. Mol Pharm 20 (7) 3589–3597. DOI
Knödler M, Buyel JF (2023) Extraction and purification of malaria vaccine candidate CLCT produced by transient expression in Nicotiana benthamiana plants. Disc Chem Eng 3, 14. DOI
Knödler M, Frank K, Kerpen L, Buyel JF (2023) Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia. Bioengineered 14 (1) 2244235. DOI
Knödler M, Opdensteinen P, Sankaranarayanan RA, Morgenroth A, Buhl EM, Mottaghy FM, Buyel JF (2023) Simple plant-based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor-targeted drug delivery and bioimaging in cancer therapy. Biotechnol Bioeng 120 (4) 1038–1054. DOI
Knödler M, Reunious PW, Buyel JF (2023) Risk assessment and bioburden evaluation of Agrobacterium tumefaciens-mediated transient protein expression in plants using the CaMV35S promoter. BMC Biotechnol 23 (1) 14. DOI
Koludarov I, Velasque M, Senoner T, Timm T, Greve C, Hamadou AB, Gupta DK, Lochnit G, Heinzinger M, Vilcinskas A, Gloag R, Harpur BA, Podsiadlowski L, Rost B, Jackson TNW, Dutertre S, Stolle E, von Reumont BM (2023) Prevalent bee venom genes evolved before the aculeate stinger wand eusociality. BMC Biol 21 (1) 229. DOI
Kottenhahn P, Philipps G, Bunk B, Spröer C, Jennewein S (2023) The restriction-modification systems of Clostridium carboxidivorans P7. Microorganisms 11 (12) 2962. DOI
Lappöhn CA, Maerz L, Stei R, Weber LG, Wolff MW (2023) Optimization and validation of analytical affinity chromatography for the in-process monitoring and quantification of peptides containing a C-tag. J Chromatogr B 1229, 123899. DOI
Lappöhn CA, Oestreich AM, Stei R, Weber LG, Maerz L, Wolff MW (2023) Process intensification for the production of a C-tagged antimicrobial peptide in Escherichia coli - First steps toward a platform technology. J Biosci Bioeng 136 (5) 358–365. DOI
Lopatriello G, Maestri S, Alfano M, Papa R, Di Vittori V, De Antoni L, Bellucci E, Pieri A, Bitocchi E, Delledonne M, Rossato M (2023) CRISPR/Cas9-mediated enrichment coupled to nanopore sequencing provides a valuable tool for the precise reconstruction of large genomic target regions. Int J Mol Sci 24 (2) 1076. DOI
Lüddecke T, Dersch L, Schulte L, Hurka S, Paas A, Oberpaul M, Eichberg J, Hardes K, Klimpel S, Vilcinskas A (2023) Functional profiling of the A-family of venom peptides from the wolf spider Lycosa shansia. Toxins 15 (5) 303. DOI
Marcolungo L, Vincenzi L, Ballottari M, Cecchin M, Cosentino E, Mignani T, Limongi A, Ferraris I, Orlandi M, Rossato M, Delledonne M (2023) Structural refinement by direct mapping reveals assembly inconsistencies near Hi-C junctions. Plants 12 (2) 320. DOI
McElroy C, Engels B, Meusinger R, Jennewein S (2023) CYPArm2 is a CYP450 monooxygenase with protoilludene 13-hydroxylase activity involved in the biosynthesis of armillyl orsellinate-type sesquiterpenoids. Chembiochem 24 (14) e202300179. DOI
Mendes SR, Gomis-Rüth FX, Goulas T (2023) Frozen fresh blood plasma preserves the functionality of native human α2-macroglobulin. Sci Rep 13, 4579. DOI
Meng LW, Yuan GR, Chen ML, Zheng LS, Dou W, Peng Y, Bai WJ, Li ZY, Vontas J, Wang JJ (2023) Cuticular competing endogenous RNAs regulate insecticide penetration and resistance in a major agricultural pest. BMC Biol 21 (1) 187. DOI
Miyai-Murai Y, Okamoto-Shibayama K, Sato T, Kikuchi Y, Kokubu E, Potempa J, Ishihara K (2023) Localization and pathogenic role of the cysteine protease dentipain in Treponema denticola. Mol Oral Microbiol 38 (3) 212–223. DOI
Muñoz-Basagoiti J, Monteiro FLL, Krumpe LRH, Armario-Najera V, Shenoy SR, Perez-Zsolt D, Westgarth HJ, Villorbina G, Bomfim LM, Raïch-Regué D, Nogueras L, Henrich CJ, Gallemí M, Moreira FRR, Torres P, Wilson J, D’arc M, Marfil S, Herlinger AL, Pradenas E, Higa LM, Otin MP, Trinité B, Twyman RM, Capell T, Tanuri A, Blanco J, Useros NI, Rech EL, Christou P, O’Keefe BR (2023) Cyanovirin-N binds to select SARS-CoV2 Spike oligosaccharides outside of the receptor binding domain and blocks infection by SARS-CoV2. Proc Natl Acad Sci USA 120 (10) e2214561120. DOI
Najafi S, Bertini E, D’Incà E, Fasoli M, Zenoni S (2023) DNA-free genome editing in grapevine using CRISPR/Cas9 ribonucleoprotein complexes followed by protoplast regeneration. Hort Res 10 (1) uhac240. DOI
Ortega-Rivera OA, Beiss V, Osota EO, Chan SK, Karan S, Steinmetz NF (2023) Production of cytoplasmic type citrus leprosis virus-like particles by plant molecular farming. Virology 578, 7–12. DOI
Ostheller ME, Balakrishnan NK, Beukenberg K, Groten R, Seide G (2023) Pilot-scale melt electrospinning of polybutylene succinate fiber mats for a biobased and biodegradable face mask. Polymers 15 (13) 2936. DOI
Pöri P, Lille M, Edelmann M, Aisala H, Santangelo D, Coda R, Sozer N (2023) Technological and sensory properties of plant-based meat analogues containing fermented sunflower protein concentrate. Future Foods 8, 100244. DOI
Rossato M, Marcolungo L, De Antoni L, Lopatriello G, Bellucci E, Cortinovis G, Frascarelli G, Nanni L, Bitocchi E, Di Vittori V, Vincenzi L, Lucchini F, Bett KE, Ramsay L, Konkin DJ, Delledonne M, Papa R (2023) CRISPR-Cas9-based repeat depletion for high-throughput genotyping of complex plant genomes. Genome Res 33 (5) 787–797. DOI
Saba-Mayoral A, Rosa C, Sobrino-Mengual G, Armario-Najera V, Christou P, Capell T (2023) Production of the SARS-CoV-2 receptor-binding domain in stably-transformed rice plants for developing country applications. Plant Biotechnol J 21 (6) 1094–1096. DOI
Sarisoy A, Acosta S, Rodríguez-Cabello JC, Czichowski P, Kopp A, Jockenhoevel S, Fernández-Colino A (2023) Bioglues based on an elastin-like recombinamer: effect of tannic acid as an additive on tissue adhesion and cytocompatibility. Int J Mol Sci 24 (7) 6776. DOI
Schick S, Groten R, Seide GH (2023) Performance spectrum of home-compostable biopolymer fibers compared to a petrochemical alternative. Polymers 15 (6) 1372. DOI
Schulte L, Damm M, Avella I, Uhrig L, Erkoc P, Schiffmann S, Fürst R, Timm T, Lochnit G, Vilcinskas A, Lüddecke T (2023) Venomics of the milos viper (Macrovipera schweizeri) unveils patterns of venom composition and exochemistry across blunt-nosed viper venoms. Front Mol Biosci 10, 1254058. DOI
Sens A, Rischke S, Hahnefeld L, Dorochow E, Schäfer SMG, Thomas D, Köhm M, Geisslinger G, Behrens F, Gurke R (2023) Pre-analytical sample handling standardization for reliable measurement of metabolites and lipids in LC-MS-based clinical research. J Mass Spectrom Adv Clin Lab 28, 35–46. DOI
Staniec D, Rut W, Drag M, Burmistrz M, Kitching M, Potempa J (2023) Mapping the substrate-binding subsite specificity of a Porphyromonas gingivalis Tpr peptidase. Acta Biochim Pol 70 (4) 963–968. DOI
Sun QZ, Li XL, Shi YF, Zhang YC, Chai WJ, Chen RY, Niu J, Wang JJ (2023) GARP: A family of glycine and alanine-rich proteins that helps spider mites feed on plants. Insect Sci 30 (5) 1337–1351. DOI
Szczęśniak K, Veillard F, Scavenius C, Chudzik K, Ferenc K, Bochtler M, Potempa J (2023)The Bacteroidetes Q-rule and glutaminyl cyclase activity increase the stability of extracytoplasmic proteins. mBio 14 (5) e00980-23. DOI
Tang Y, Zhang Z, Yang Z, Wu J (2023) CRISPR/Cas9 and Agrobacterium tumefaciens virulence proteins synergistically increase efficiency of precise genome editing via homology directed repair in plants. J Exp Bot 74 (12) 3518–3530. DOI
Tarrés-Solé A, Battistini F, Gerhold JM, Piétrement O, Martínez-García B, Ruiz-López E, Lyonnais S, Bernadó P, Roca J, Orozco M, Le Cam E, Sedman J, Solà M (2023) Structural analysis of the Candida albicans mitochondrial DNA maintenance factor Gcf1p reveals a dynamic DNA-bridging mechanism. Nucleic Acids Res 51 (11) 5864–5882. DOI
Toffali L, D'Ulivo B, Giagulli C, Montresor A, Zenaro E, Delledonne M, Rossato M, Iadarola B, Sbarbati A, Bernardi P, Angelini G, Rossi B, Lopez N, Linke WA, Unger A, Di Silvestre D, Benazzi L, De Palma A, Motta S, Constantin G, Mauri P, Laudanna C (2023) An isoform of the giant protein titin is a master regulator of human T lymphocyte trafficking. Cell Rep 42 (5) 112516. DOI
Vocadlova K, Lamp B, Benes K, Matha V, Lee KZ, Vilcinskas A (2023) Crude extracts of Talaromyces strains (Ascomycota) affect honey bee (Apis mellifera) resistance to chronic bee paralysis virus. Viruses 15 (2) 343. DOI
Vocadlova K, Lüddecke T, Patras MA, Marner M, Hartwig C, Benes K, Matha V, Mraz P, Schäberle TF, Vilcinskas A (2023) Extracts of Talaromyces purpureogenus strains from Apis mellifera bee bread inhibit the growth of Paenibacillus spp. in vitro. Microorganisms 11 (8) 2067. DOI
Wang D, Yu Z, Guan M, Cai Q, Wei J, Ma P, Xue Z, Ma R, Oksman-Caldentey KM, Rischer H (2023) Comparative transcriptome analysis of Veratrum maackii and Veratrum nigrum reveals multiple candidate genes involved in steroidal alkaloid biosynthesis. Sci Rep 13 (1) 8198. DOI
Wang ZG, Chen RY, Jiang YK, Wang ZW, Wang JJ, Niu J (2023) Investigation of potential non-target effects to a ladybeetle Propylea japonica in the scenario of RNAi-based pea aphid control. Entomol Gen 43 (1) 79–88. DOI
Wielento A, Bereta GP, Szczęśniak K, Jacuła A, Terekhova M, Artyomov MN, Hasegawa Y, Grabiec AM, Potempa J (2023) Accessory fimbrial subunits and PPAD are necessary for TLR2 activation by Porphyromonas gingivalis. Mol Oral Microbiol 38 (4) 334–346. DOI
Windfelder AG, Steinbart J, Flögel U, Scherberich J, Kampschulte M, Krombach GA, Vilcinskas A (2023) A quantitative micro-tomographic gut atlas of the lepidopteran model insect Manduca sexta. iScience 26 (6) 106801. DOI
Wolters SM, Benninghaus VA, Roelfs KU, van Deenen N, Twyman RM, Prüfer D, Schulze Gronover C (2023) Overexpression of a pseudo-etiolated-in-light-like protein in Taraxacum koksaghyz leads to a pale green phenotype and enables transcriptome-based network analysis of photomorphogenesis and isoprenoid biosynthesis. Front Plant Sci 14, 1228961. DOI
Wu D, Mehdipour AR, Finke F, Goojani HG, Groh RR, Grund TN, Reichhart TMB, Zimmermann R, Welsch S, Bald D, Shepherd M, Hummer G, Safarian S (2023) Dissecting the conformational complexity and mechanism of a bacterial heme transporter. Nature Chem Biol 19 (8) 992–1003. DOI
Xu L, Jiang HB, Yu JL, Pan D, Tao Y, Lei Q, Chen Y, Liu Z, Wang JJ (2023) Two odorant receptors regulate 1-octen-3-ol induced oviposition behavior in the oriental fruit fly. Commun Biol 6 (1) 176. DOI
You Y, Bai C, Wang W, Zhan T, Hu X, Hao F, Xia M, Liu Y, Ma T, Liu Y, Zheng C, Pu T, Zhang Y, Lu Y, Ding N, Li J, Yin Y, Chen Y, Wang L, Zhou J, Niu L, Xiu Y, Lu Y, Jia T, Liu X, Zhang C (2023) Comparative proteomics in captive giant pandas to identify proteins involved in age-related cataract formation.. Sci Rep 13 (1) 12722. DOI
Zaza G, Neri F, Bruschi M, Granata S, Petretto A, Bartolucci M, di Bella C, Candiano G, Stallone G, Gesualdo L, Furian L (2023) Proteomics reveals specific biological changes induced by the normothermic machine perfusion of donor kidneys with a significant up-regulation of Latexin. Sci Rep 13 (1) 5920. DOI
Zhang Q, Dou W, He LQ, Yu SS, Chen JQ, Zheng LY, Wang L, Smagghe G, Wang JJ (2023) Pannier is a key regulator of embryogenesis, pupal development and female reproduction in the insect pest Bactrocera dorsalis. Pest Manag Sci 79 (4) 1352–1361. DOI
Zhou XR, Beier S, Brilhaus D, Martins Rodrigues C, Mühlhaus T, von Suchodoletz D, Twyman RM, Usadel B, Kranz A (2023) DataPLAN: A web-based data management plan generator for the plant sciences. Data 8 (11) 159. DOI
Zorzi G, Gambini S, Negri S, Guzzo F, Commisso M (2023) Untargeted metabolomics analysis of the orchid species Oncidium sotoanum reveals the presence of rare bioactive C-diglycosylated chrysin derivatives. Plants 12 (3) 655. DOI
Preprints
Bhattacharya A, Mukherjee R, Kuncha SK, Brunstein M, Rathore R, Junek S, Münch C, Dikic I (2023) TBC1D15 potentiates lysosomal regeneration from damaged membranes. bioRxiv 2022.12.14.520480. DOI
Cortinovis G, Vincenzi L, Anderson R, Marturano G, Marsh JI, Bayer PE, Rocchetti L, Frascarelli G, Lanzavecchia G, Pieri A, Benazzo A, Bellucci E, Di Vittori V, Nanni L, Ferreira Fernández JJ, Rossato M, Aguilar OM, Morrell PL, Rodriguez M, Gioia T, Neumann K, Alvarez Diaz JC, Gratias-Weill A, Klopp C, Geffroy V, Bitocchi E, Delledonne M, Edwards D, Papa R (2023) Adaptive gene loss in the common bean pan-genome during range expansion and domestication. bioRxiv 2023.11.23.568464. DOI
Frascarelli G, Galise TR, D’Agostino N, Cafasso D, Cozzolino S Cortinovis G, Sparvoli F, Bellucci E, Rossato M, Vincenzi L, Benazzo A, Delledonne M, Bitocchi E, Papa R (2023) The evolutionary history of the common bean (Phaseolus vulgaris) as revealed by chloroplast and nuclear genome analysis. bioRxiv 2023.06.09.544374. DOI
Mäckelmann S, Känel A, Kösters LM, Lyko P, Prüfer D, Noll GA, Wicke S (2023) Gene complementation analysis suggests that dodder plants (Cuscuta spp.) do not depend on the host FT protein for flowering. bioRxiv 2022.12.19.520981. DOI
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Preprints
Koludarov I, Velasque M, Timm T, Greve C, Hamadou AB, Gupta DK, Lochnit G, Heinzinger M, Vilcinskas A, Gloag R, Harpur BA, Podsiadlowski L, Rost B, Jackson TNW, Dutertre S, Stolle E, von Reumont BM (2022) A common venomous ancestor? Prevalent bee venom genes evolved before the aculeate stinger while few major toxins are bee-specific. bioRxiv 2022.01.21.477203. DOI
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Röder J, Fischer R, Commandeur U (2107) Engineering Potato virus X particles for a covalent protein based attachment of enzymes. Small 13 (48) 1702151. DOI
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Buyel JF, Opdensteinen P, Fischer R (2015) Cellulose-based filter aids increase the capacity of depth filters during the downstream processing of plant-derived biopharmaceutical proteins. Biotechnol J 10 (4) 584–591. DOI
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Chocarro C, Lloveras J (2015) The effect of row spacing on alfalfa seed and forage production under irrigated Mediterranean agricultural conditions. Grass Forage Sci 70 (4) 651–660. DOI
Cremer C, Braun H, Mladenov R, Hein L, Cong X, Jost E, Brümmendorf TH, Fischer R, Carloni P, Barth S, Nachreiner T (2015) Novel angiogenin mutants with increased cytotoxicity enhance the depletion of pro-inflammatory macrophages and leukemia cells ex vivo. Cancer Immunol Immunother 64 (12) 1575–1586. DOI
Cremer C, Vierbuchen T, Hein L, Fischer R, Barth S, Nachreiner T (2015) Angiogenin mutants as novel effector molecules for the generation of fusion proteins with increased cytotoxic potential. J Immunother 38 (3) 85–95. DOI
Dickmeis C, Honickel MM, Fischer R, Commandeur U (2015) Production of hybrid chimeric PVX particles using a combination of TMV and PVX-based expression vectors. Front Bioeng Biotechnol 3, 189. DOI
Epping J, van Deenen N, Niephaus E, Stolze A, Fricke J, Huber C, Eisenreich W, Twyman RM, Prüfer D, Schulze Gronover C (2015) A rubber transferase activator is necessary for natural rubber biosynthesis in dandelion. Nature Plants 1, 15048. DOI
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Fitting J, Blume T, Ten Haaf A, Blau W, Gattenlöhner S, Tur MK, Barth S (2015) Generation of novel internalizing antibody fragments by phage display for immunotoxin-based treatment of acute myeloid leukemia. Monoclon Antib 7 (2) 390–402. DOI
Fritsch L, Fischer R, Schillberg S, Wamback C, Schröper F (2015) Next-generation sequencing is a robust strategy for the detection of zygosity in transgenic maize. Transgenic Res 24 (4) 615–623. DOI
Kaiser M, Pereira S, Pohl L, Ketelhut, Kemper B, Gorzelanny, Galla HJ, Moerschbacher BM, Goycoolea FM (2015) Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells. Sci Rep 5, 10048. DOI
Kapelski S, Boes A, Spiegel H, de Almeida M, Klockenbring T, Reimann A, Fischer R, Barth S, Fendel R (2015) Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody. Malaria J 14, 50. DOI
Kapelski S, de Almeida M, Fischer R, Barth S, Fendel R (2015) Antimalarial activity of granzyme B and its targeted delivery by a granzyme B–single-chain Fv fusion protein. Antimicrob Agents Chemother 59 (1) 669–672. DOI
Klinger J, Fischer R, Commandeur U (2015) Comparison of Thermobifida fusca cellulases expressed in Escherichia coli and Nicotiana tabacum indicates advantages of the plant system for the expression of bacterial cellulases. Front Plant Sci 6, 1047. DOI
Knorr E, Schmidtberg H, Arslan D, Bingsohn L, Vilcinskas A (2015) Translocation of bacteria from the gut to the eggs triggers maternal transgenerational immune priming in Tribolium castaneum. Biol Lett 11 (12) 20150885. DOI
Hamer SN, Cord-Landwehr S, Biarnés X, Planas A, Waegeman H, Moerschbacher BM, Kolkenbrock S (2015) Enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases. Sci Rep 5, 8716. DOI
Hristodorov D, Mladenov R, von Felbert V, Huhn M, Fischer R, Barth S, Thepen T (2015) Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies. Monoclon Antib 6 (5) 853–862. DOI
Laibach N, Hillebrand A, Twyman RM, Prüfer D, Schulze Gronover C (2015) Identification of a rubber elongation factor protein in Taraxacum brevicorniculatum that is localized on rubber particles and promotes rubber biosynthesis. Plant J 82 (4) 609–620. DOI
Lampis S, Santi C, Ciurli A, Andreolli M, Vallini G (2015) Promotion of arsenic phytoextraction efficiency in the fern Pteris vittata by the inoculation of As-resistant bacteria: a soil bioremediation perspective. Front Plant Sci 6, 80. DOI
Leufken CM, Moerschbacher BM, Dirks-Hofmeister ME (2015) Dandelion PPO-1/PPO-2 domain-swaps: The C-terminal domain modulates the pH optimum and the linker affects SDS-mediated activation and stability. Biochim Biophys Acta 1854 (2) 178–186. DOI
Ma JKC, Drossard J, Lewis D, Altmann F, Boyle J, Christou P, Cole T, Dale P, van Dolleweerd CJ, Isitt V, Katinger D, Lobedan M, Mertens H, Paul MJ, Rademacher T, Sack M, Hundleby PAC, Stiegler G, Stoger E, Twyman RM, Vcelar B, Fischer R (2015) Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants. Plant Biotechnol J 13 (8) 1106–1120. DOI
Madhuprakash J, El Gueddari NE, Moerschbacher BM, Podile AR (2015) Catalytic efficiency of chitinase-D on insoluble chitinous substrates was improved by fusing auxiliary domains. PLoS One 10 (1) e0116823. DOI
Madhuprakash J, El Gueddari NE, Moerschbacher BM, Podile AR (2015) Production of bioactive chitosan oligosaccharides using the hypertransglycosylating chitinase-D from Serratia proteamaculans. Bioresource Technol 198, 503–509. DOI
Maskus DJ, Bethke S, Seidel M, Kapelski S, Addai-Mensah O, Boes A, Edgü G, Spiegel H, Reimann A, Fischer R, Barth S, Klockenbring T, Fendel R (2015) Isolation, production and characterization of fully human monoclonal antibodies directed to Plasmodium falciparum MSP10. Malar J 14, 276. DOI
Menchicchi B, Fuenzalida JP, Hensel A, Swamy MJ, David L, Rochas C, Goycoolea FM (2015) Biophysical analysis of the molecular interactions between polysaccharides and mucin. Biomacromolecules 16 (3) 924–935. DOI
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Milia G, Camiolo S, Avesani L, Porceddu A (2015) The dynamic loss and gain of introns during the evolution of the Brassicaceae. Plant J 82 (6) 915–924. DOI
Mladenov R, Hristodorov D, Thepen T, Cremer C, Hein L, Niesen J, Brehm H, Brümmendorf T, Jost E, Fischer R, Stockmeyer B, Barth S, Stein C (2015) The Fc-alpha receptor is a new immunotherapeutic target antigen for the treatment of myeloid leukaemia. Int J Cancer 137 (11) 2729–2738. DOI
Niesen J, Brehm H, Stein C, Berges N, Pardo A, Fischer R, Ten Haaf A, Gattenlöhner S, Tur MK, Barth S (2015) In vitro effects and ex vivo binding of an EGFR-specific immunotoxin on rhabdomyosarcoma cells. J Cancer Res Clin Oncol 141 (6) 1049–1061. DOI
Niesen J, Stein C, Brehm H, Hehmann-Titt G, Fendel R, Melmer G, Fischer R, Barth S (2015) Novel EGFR-specific immunotoxins based on panitumumab and cetuximab show in vitro and ex vivo activity against different tumor entities. J Cancer Res Clin Oncol 141 (12) 2079–2095. DOI
O'Keeffe C, Champion S, Allsop D (2015) Demonstrating the effect of forensic firearm countermeasures: Bullet characteristics generated due to barrel modifications. Forensic Sci Int 257, 13–19. DOI
Popovic M, Prodanovic R, Ostafe R, Schillberg S, Fischer R, Gavrovic-Jankulovic M (2015) Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens. Immunologic Res 61 (3) 230–239. DOI
Pöppel AK, Vogel H, Wiesner J, Vilcinskas A (2015) Combinatorial activities of antimicrobial peptides expressed in medicinal maggots of the blow fly Lucilia sericata against bacteria. Antimicrob Agents Chemother 59 (9) 2508–2514. DOI
Rahnamaeian M, Cytrynska M, Zdybicka-Barabas A, Dobslaff K, Twyman RM, Züchner T, Sadd B, Regoes R, Schmid-Hempel P, Vilcinskas A (2015) Insect antimicrobial peptides show potentiating functional interactions against Gram-negative bacteria. Proc R Soc B 282 (1806) 20150293. DOI
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Sack M, Rademacher T, Spiegel H, Boes A, Hellwig S, Drossard J, Stoger E, Fischer R (2015) From gene to harvest: Insights into upstream process development for the GMP production of a monoclonal antibody in transgenic tobacco plants. Plant Biotechnol J 13 (8) 1094–1105. DOI
Santos-Carballal B, Aaldering LJ, Ritzefeld M, Pereira S, Sewald N, Moerschbacher BM, Götte M, Goycoolea FM (2015) Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells. Sci Rep 5, 13567. DOI
Schlich K, Hund-Rinke K (2015) Influence of soil properties on the effect of silver nanomaterials on microbial activity in five soils. Environ Poll 196 (1) 321–330. DOI
Schott M, Klein B, Vilcinskas A (2015) Sniffer bees: detection of illicit drugs by trained honeybees (Apis mellifera). PLoS One 10 (6) e0128528. DOI
Schubert M, Houdelet M, Kogel KH, Fischer R, Schillberg S, Nölke G (2015) Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays). Transgenic Res 24 (5) 885–895. DOI
Shukla S, DiFranco NA, Wen AM, Commandeur U, Steinmetz NF (2015) To target or not to target: active vs. passive tumor homing of filamentous nanoparticles based on Potato virus X. Cel Mol Bioeng 8 (3) 433–444. DOI
Shukla S, Eber FJ, Nagarajan AS, DiFranco NA, Schmidt N, Wen AM, Eiben S, Twyman RM, Wege C, Steinmetz NF (2015) The impact of aspect ratio on the biodistribution and tumor homing of rigid soft-matter nanorods. Adv Healthc Mater 4 (6) 874–882. DOI
Sonnack L, Kampe S, Muth-Köhne E, Erdinger L, Henny N, Hollert H, Schäfers C, Fenske M (2015) Effects of metal exposure on motor neuron development, neuromasts and the escape response of zebrafish embryos. Neurotoxicol Teratol 50, 33–42. DOI
Spohner SC, Zahn D, Schaum V, Quitmann H, Czermak P (2015) Recombinant α-L-rhamnosidase from Aspergillus terreus in selective trimming of α-L-rhamnose from steviol glycosides. J Mol Cat B: Enzymatic 122 (12) 248–254. DOI
Spiegel H, Boes A, Kastilan R, Kapelski S, Edgue G, Beiss V, Chubodova I, Schillberg S, Reimann A, Fischer R (2015) The stage-specific in vitro efficacy of a malaria antigen cocktail provides valuable insights into the development of effective multi-stage vaccines. Biotechnol J 10 (10) 1651–1659. DOI
Spiegel H, Boes A, Voepel N, Beiss V, Edgue G, Rademacher T, Sack M, Schillberg S, Reimann A, Fischer R (2015) Application of a scalable plant transient gene expression platform for malaria vaccine development. Front Plant Sci 6, 1169. DOI
Spiegel H, Schinkel H, Kastilan R, Dahm P, Boes A, Scheuermayer M, Chudobová I, Maskus D, Fendel R, Schillberg S, Reimann A, Fischer R (2015) Optimization of a multi-stage, multi-subunit malaria vaccine candidate for the production in Pichia pastoris by the identification and removal of protease cleavage sites. Biotechnol Bioeng 112 (4) 659–667. DOI
Stöveken J, Singh R, Kolkenbrock S, Zakrzewski M, Wibberg D, Eikmeyer FG, Pühler A, Schlüter A, Moerschbacher BM (2015) Successful heterologous expression of a novel chitinase identified by sequence analyses of the metagenome from a chitin-enriched soil sample. J Biotechnol 201, 60–68. DOI
Ten Haaf A, Pscherer S, Fries K, Barth S, Gattenlöhner S, Tur MK (2015) Phage display-based on-slide selection of tumor-specific antibodies on formalin-fixed paraffin-embedded human tissue biopsies. Immunol Lett 166 (2) 65–78. DOI
Tinazzi E, Merlin M, Bason C, Beri R, Zampieri R, Lico C, Bartoloni E, Puccetti A, Lunardi C, Pezzotti M, Avesani L (2015) Plant-derived chimeric virus particles for the diagnosis of primary Sjögren syndrome. Front Plant Sci 6, 1080. DOI
Tonk M, Cabezas-Cruz A, Valdés JJ, Rego RO, Grubhoffer L, Estrada-Peña A, Vilcinskas A, Kotsyfakis M, Rahnamaeian M (2015) Ixodes ricinus defensins attack distantly-related pathogens. Dev Comp Immunol 53 (2) 358–365. DOI
Tonk M, Knorr E, Cabezas-Cruz A, Valdés JJ, Kollewe C, Vilcinskas A (2015) Tribolium castaneum defensins are primarily active against Gram-positive bacteria. J Invertebrate Pathol 132, 208–215. DOI
Valdés O, Vergara CE, Camarada MB, Carrasco-Sánchez V, Nachtigall FM, Tapia J, Fischer R, González-Nilo FD, Santos LS. Synthesis and characterization of an insoluble polymer based on polyamidoamine: applications for the decontamination of metals in aqueous systems. J Environ Managemt 147, 321–329. DOI
van Rijn P, van Bezouwen LS, Fischer R, Boekema EJ, Böker A, Commandeur U (2015) Virus-SiO2 and virus-SiO2-Au hybrid particles with tunable morphology. Part Part Syst Charact 32 (1) 43–47. DOI
Will T, Vilcinskas A (2015) The structural sheath protein of aphids is required for phloem feeding. Insect Biochem Mol Biol 57, 34–40. DOI
Zenaro E, Pietronigro E, Bianca VD, Piacentino G, Marongiu L, Budui S, Turano E, Rossi B, Angiari S, Dusi S, Montresor A, Carlucci T, Nanì S, Tosadori G, Calciano L, Catalucci D, Berton G, Bonetti B, Constantin G (2015) Neutrophils promote Alzheimer's disease-like pathology and cognitive decline via LFA-1 integrin. Nature Med 21 (8) 880–886. DOI
Angiari S, Donnarumma T, Rossi B, Dusi S, Pietronigro E, Zenaro E, Della Bianca V, Toffali L, Piacentino G, Budui S, Rennert P, Xiao S, Laudanna C, Casasnovas JM, Kuchroo VK, Constantin G (2014) TIM-1 glycoprotein binds the adhesion receptor P-selectin and mediates T cell trafficking during inflammation and autoimmunity. Immunity 40 (4) 542–553. DOI
Aragona M, Minio A, Ferrarini A, Valente MT, Bagnaresi P, Orrù L, Tononi P, Zamperin G, Infantino A, Valè G, Cattivelli L, Delledonne M (2014) De novo genome assembly of the soil-borne fungus and tomato pathogen Pyrenochaeta lycopersici. BMC Genomics 15, 313.
Avesani L, Merlin M, Gecchele E, Capaldi S, Brozzetti A, Falorni A, Pezzotti M (2014) Comparative analysis of different biofactories for the production of a major diabetes autoantigen. Transgenic Res 23 (2) 281–291.
Bai C, Rivera SM, Medina V, Alves R, Vilaprinyo E, Sorribas A, Canela R, Capell T, Sandmann G, Christou P, Zhu C (2014) An in vitro system for the rapid functional characterization of genes involved in carotenoid biosynthesis and accumulation. Plant J 77 (3) 464–475.
Berges N, Hehmann-Titt G, Hristodorov D, Melmer G, Thepen T, Barth S (2014) Human cytolytic fusion proteins: modified versions of human granzyme B and angiogenin have the potential to replace bacterial toxins in targeted therapies against CD64+ diseases. Antibodies 3 (1) 92–115.
Bialon M, Schellenberg L, Herzog N, Kraus S, Jörißen H, Fischer R, Stein C, Nähring J, Barth S, Püttmann C (2014) Cloning murine antibody V-genes with non-degenerate primers and conversion to a recombinant antibody format. Monoclon Antib Immunodiagn Immunother 33 (6) 369–377.
Blohm L, Püttmann C, Holz S, Piechotta G, Albers J, Dammers C, Kleines M, Krüttgen A, Melmer G, Nähring J, Barth S, Nebling E (2014) Rapid detection of different human anti-HCV immunoglobulins on electrical biochips. Antib Technol J 2014 (4) 23–32.
Brehm H, Niesen J, Mladenov R, Stein C, Pardo A, Fey G, Helfrich W, Fischer R, Gattenlöhner S, Barth S (2014) A CSPG4-specific immunotoxin kills rhabdomyosarcoma cells and binds to primary tumor tissues. Cancer Lett 352 (2) 228–235.
Breitenbach J, Bai C, Rivera SM, Canela R, Capell T, Christou P, Zhu C, Sandmann G (2014) A novel carotenoid, 4-keto-α-carotene, as an unexpected by-product during genetic engineering of carotenogenesis in rice callus. Phytochemistry 98, 85–91.
Bruckman MA, Randolph LN, Vanmeter A, Hern S, Shoffstall AJ, Taurog RE, Steinmetz NF (2014) Biodistribution, pharmacokinetics, and blood compatibility of native and PEGylated Tobacco mosaic virus nano-rods and -spheres in mice. Virology 449, 163–173.
Buntru M, Vogel S, Spiegel H, Schillberg S (2014) Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system. BMC Biotechnol 14 (1) 37.
Buyel JF, Gruchow HM, Boes A, Fischer R (2014) Rational design of a host cell protein heat precipitation step can simplify the subsequent purification of recombinant proteins from tobacco. Biochem Eng J 88, 162–170.
Buyel JF, Fischer R (2014) Characterization of complex systems using the design of experiments approach: transient protein expression in tobacco as a case study. J Vis Exp 83, e51216.
Buyel JF, Fischer R (2014) Downstream processing of biopharmaceutical proteins produced in plants: The pros and cons of flocculants. Bioengineered 5 (2) 138–142.
Buyel JF, Fischer R (2014) Flocculation increases the efficacy of depth filtration during the downstream processing of recombinant pharmaceutical proteins produced in tobacco. Plant Biotechnol J 12 (2) 240–252.
Buyel JF, Fischer R (2014) Generic chromatography-based purification strategies accelerate the development of downstream processes for biopharmaceutical proteins produced in plants. Biotechnol J 9 (4) 566–577.
Buyel JF, Fischer R (2014) Scale-down models to optimize a filter train for the downstream purification of recombinant pharmaceutical proteins produced in tobacco leaves. Biotechnol J 9 (3) 415–425.
Cavallini E, Zenoni S, Finezzo L, Guzzo F, Zamboni A, Avesani L, Tornielli GB (2014) Functional diversification of grapevine MYB5a and MYB5b in the control of flavonoid biosynthesis in a petunia anthocyanin regulatory mutant. Plant Cell Physiol 55 (3) 517–534.
Company N, Nadal A, La Paz JL, Martínez S, Rasche S, Schillberg S, Montesinos E, Pla M (2014) The production of recombinant cationic α-helical antimicrobial peptides in plant cells induces the formation of protein bodies derived from the endoplasmic reticulum. Plant Biotechnol J 12 (1) 81–92.
Dickmeis C, Fischer R, Commandeur U (2014) Potato virus X-based expression vectors are stabilized for long-term production of proteins and larger inserts. Biotechnol J 9 (11) 1369–1379.
Dirks-Hofmeister ME, Singh R, Leufken CM, Inlow JK, Moerschbacher BM (2014) Structural diversity in the dandelion (Taraxacum officinale) polyphenol oxidase family results in different responses to model substrates. PLoS One 9 (6) e99759.
Freitak D, Schmidtberg H, Dickel F, Lochnit G, Vogel H, Vilcinskas A (2014) The maternal transfer of bacteria can mediate trans-generational immune priming in insects. Virulence 5 (4) 547–554.
Fuenzalida JP, Weikert T, Hoffmann S, Vila-Sanjurjo C, Moerschbacher BM, Goycoolea FM, Kolkenbrock S (2014) Affinity protein-based FRET tools for cellular tracking of chitosan nanoparticles and determination of the polymer degree of acetylation. Biomacromolecules 15 (7) 2532–2539.
Gasch T, Vilcinskas A (2014) The chemical defense in larvae of the earwig Forficula auricularia. J Insect Physiol 67, 1–8.
Garvey M, Klinger J, Klose H, Fischer R, Commandeur U (2014) Expression of recombinant cellulase Cel5A from Trichoderma reesei in tobacco plants. J Vis Exp 88, e51711.
Gecchele E, Schillberg S, Merlin M, Pezzotti M, Avesani L (2014) A downstream process allowing the efficient isolation of a recombinant amphiphilic protein from tobacco leaves. J Chromatogr B 960, 34–42.
Gökçen A, Vilcinskas A, Wiesner J (2014) Biofilm-degrading enzymes from Lysobacter gummosus. Virulence 5 (3) 378–387.
Groscurth S, Müller B, Visser F, Blob B, Menzel M, Rüping BA, Twyman RM, Prüfer D, Noll G (2014) Uncertain role of MtSEO-F3 in assembly of Medicago truncatula forisomes. Plant Signal Behav 9 (9) e29581.
Häkkinen ST, Raven N, Henquet M, Laukkanen ML, Anderlei T, Pitkänen JP, Twyman RM, Bosch D, Oksman-Caldentey KM, Schillberg S, Ritala A (2014) Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody. Biotechnol Bioeng 111 (2) 336–346.
Havenith H, Raven N, Di Fiore S, Fischer R, Schillberg S (2014) Image-based analysis of cell-specific productivity for plant cell suspension cultures. Plant Cell Tiss Org Cult 117 (3) 393–399.
Honke N, Ohl K, Wiener A, Bierwagen J, Peitz J, Di Fiore S, Fischer R, Wagner N, Wüller S, Tenbrock K (2014) The p38-mediated rapid down-regulation of cell surface gp130 expression impairs interleukin-6 signaling in the synovial fluid of juvenile idiopathic arthritis patients. Arthritis Rheumatol 66 (2) 470–478.
Hofbauer A, Peters J, Arcalis E, Rademacher T, Lampel J, Eudes F, Vitale A, Stoger E (2014) The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27-kDa γ-zein sequence. Frontiers Bioeng Biotechnol 2, 67.
Hristodorov D, Mladenov R, Brehm H, Fischer R, Barth S, Thepen T (2014) Recombinant H22(scFv) blocks CD64 and prevents the capture of anti-TNF monoclonal antibody: A potential strategy to enhance anti-TNF therapy. Monoclon Antib 6 (5) 1283–1289.
Hristodorov D, Nordlohne J, Mladenov R, Huhn M, Fischer R, Thepen T, Barth S (2014) Human microtubule-associated protein tau mediates targeted killing of CD30+ lymphoma cells in vitro and inhibits tumour growth in vivo. Br J Haematol 164 (2) 251–257.
Hund-Rinke K, Schlich K (2014) The potential benefits and limitations of different test procedures to determine the effects of Ag nanomaterials and AgNO3 on microbial nitrogen transformation in soil. Env Sci Eur 26 (1) 28.
Ibl V, Kapusi E, Arcalis E, Kawagoe Y, Stoger E (2014) Fusion, rupture, and degeneration: the fate of in vivo-labelled PSVs in developing barley endosperm. J Exp Bot 65 (12) 3249–3261.
Ibl V, Stöger E (2014) Live cell imaging during germination reveals dynamic tubular structures derived from protein storage vacuoles of barley aleurone cells. Plants 3 (3) 442–457.
Jansen F, Gillessen B, Mueller F, Commandeur U, Fischer R, Kreuzaler F (2014) Metabolic engineering for p-coumaryl alcohol production in Escherichia coli by introducing an artificial phenylpropanoid pathway. Biotechnol Appl Biochem 61 (6) 646–654.
Juodeikiene G, Vidmantiene D, Basinskiene L, Cernauskas D, Klupsaite D, Petrauskas A, Bartkiene E, de Koe WJ (2014) Recent advances in the rapid acoustic screening of deoxynivalenol in wheat grains. World Mycotox J 7 (4) 517–525.
Kapelski S, Klockenbring T, Fischer R, Barth S, Fendel R (2014) Assessment of the neutrophilic antibody-dependent respiratory burst (ADRB) response to Plasmodium falciparum. J Leukoc Biol 96 (6) 1131–1142.
Lin Y, Lu JJ, Wu MD, Zhou MB, Fang W, Ide Y, Tang DQ (2014) Identification, cross-taxon transferability and application of full-length cDNA SSR markers in Phyllostachys pubescens. SpringerPlus 3, 486.
Livi CM, Blanzieri E (2014) Protein-specific prediction of mRNA binding using RNA sequences, binding motifs and predicted secondary structures. BMC Bioinformatics 15 (1) 123.
Lovato A, Faoro F, Gambino G, Maffi D, Bracale M, Polverari A, Santi L (2014) Construction of a synthetic infectious cDNA clone of Grapevine Algerian latent virus(GALV-Nf) and its biological activity in Nicotiana benthamiana and grapevine plants. Virology J 11 (1) 186.
Manara A, Dalcorso G, Leister D, Jahns P, Baldan B, Furini A (2014) AtSIA1 and AtOSA1: two Abc1 proteins involved in oxidative stress responses and iron distribution within chloroplasts. New Phytol 201 (2) 452–465.
Mandal MK, Fischer R, Schillberg S, Schiermeyer A (2014) Inhibition of protease activity by antisense RNA improves recombinant protein production in Nicotiana tabacum cv Bright Yellow 2 (BY-2) suspension cells. Biotechnol J 9 (8) 1065–1073.
Masani MY, Noll GA, Parveez GK, Sambanthamurthi R, Prüfer D (2014) Efficient transformation of oil palm protoplasts by PEG-mediated transfection and DNA microinjection. PLoS One 9 (5) e96831.
Menchicchi B, Fuenzalida JP, Bobbili KB, Hensel A, Swamy MJ, Goycoolea FM (2014) The structure of chitosan determines its interactions with mucin. Biomacromolecules 15 (10) 3550–3558.
Miettinen K, Dong L, Navrot N, Schneider T, Burlat V, Pollier J, Woittiez L, van der Krol S, Lugan R, Ilc T, Verpoorte R, Oksman-Caldentey KM, Martinoia E, Bouwmeester H, Goossens A, Memelink J, Werck-Reichhart D (2014) The seco-iridoid pathway from Catharanthus roseus. Nature Commun 5, 3606.
Mukherjee K, Vilcinskas A (2014) Development and immunity-related microRNAs of the lepidopteran model host Galleria mellonella. BMC Genomics 15 (1) 705.
Müller B, Groscurth S, Menzel M, Rüping BA, Twyman RM, Prüfer D, Noll GA (2014) Molecular and ultrastructural analysis of forisome subunits reveals the principles of forisome assembly. Ann Bot 113 (7) 1121–1137.
Nölke G, Houdelet M, Kreuzaler F, Peterhänsel C, Schillberg S (2014) The expression of a recombinant glycolate dehydrogenase polyprotein in potato (Solanum tuberosum) plastids strongly enhances photosynthesis and tuber yield. Plant Biotechnol J 12 (6) 734–742.
Ostafe R, Prodanovic R, Nazor J, Fischer R (2014) Ultra-high-throughput screening method for the directed evolution of glucose oxidase. Chem Biol 21 (3) 414–421.
Ostafe R, Prodanovic R, Ung WL, Weitz DA, Fischer R (2014) A high-throughput cellulase screening system based on droplet microfluidics. Biomicrofluidics 8, 041102.
Palumbo MC, Zenoni S, Fasoli M, Massonnet M, Farina L, Castiglione F, Pezzotti M, Paci P (2014) Integrated network analysis identifies fight-club nodes as a class of hubs encompassing key putative switch genes that induce major transcriptome reprogramming during grapevine development. Plant Cell 26 (12) 4617–4635.
Peremarti A, Marè C, Aprile A, Roncaglia E, Cattivelli L, Villegas D, Royo C (2014) Transcriptomic and proteomic analyses of a pale-green durum wheat mutant shows variations in photosystem components and metabolic deficiencies under drought stress. BMC Genomics 15, 125.
Peuscher A, Gassler N, Schneider U, Thom P, Rasche S, Spiegel H, Schillberg S (2014) An immunohistochemical assay on human tissue using a human primary antibody. J Immunoassay Immunochem 35 (3) 322–334.
Pilati S, Brazzale D, Guella G, Milli A, Ruberti C, Biasioli F, Zottini M, Moser C (2014) The onset of grapevine berry ripening is characterized by ROS accumulation and lipoxygenase-mediated membrane peroxidation in the skin. BMC Plant Biol 14, 87.
Pöppel AK, Koch A, Kogel KH, Vogel H, Kollewe C, Wiesner J, Vilcinskas A (2014) Lucimycin, an antifungal peptide from the therapeutic maggot of the common green bottle fly Lucilia sericata. Biol Chem 395 (6) 649–656.
Post J, Eisenreich W, Huber C, Twyman RM, Prüfer D, Schulze Gronover C (2014) Establishment of an ex vivo laticifer cell suspension culture from Taraxacum brevicorniculatum as a production system for cis-isoprene. J Mol Cat B 103, 85–93.
Ritala A, Dong L, Imseng N, Seppänen-Laakso T, Vasilev N, van der Krol S, Rischer H, Maaheimo H, Virkki A, Brändli J, Schillberg S, Eibl R, Bouwmeester H, Oksman-Caldentey KM (2014) Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway. J Biotechnol 176, 20–28.
Rocha S, Monjardino P, Mendonça D, da Câmara Machado A, Fernandes R, Sampaio P, Salema R (2014) Lignification of developing maize (Zea mays L.) endosperm transfer cells and starchy endosperm cells. Frontiers Plant Sci 5, 102.
Schiffer S, Rosinke R, Jost E, Hehmann-Titt G, Huhn M, Melmer G, Barth S, Thepen T (2014) Targeted ex vivo reduction of CD64-positive monocytes in chronic myelomonocytic leukemia and acute myelomonocytic leukemia using human granzyme B-based cytolytic fusion proteins. Int J Cancer 135 (6) 1497–1508.
Schiller V, Zhang X, Hecker M, Schäfers C, Fischer R, Fenske M (2014) Species-specific considerations in using the fish embryo test as an alternative to identify endocrine disruption. Aquat Toxicol 155 (10) 62–72.
Schramm OG, López-Cortés X, Santos LS, Laurie VF, González Nilo FD, Krolik M, Fischer R, Di Fiore S (2014) pH-dependent nano-capturing of tartaric acid using dendrimers. Soft Matter 10 (4) 600–608.
Shukla S, Dickmeis C, Nagarajan AS, Fischer R, Commandeur U, Steinmetz NF (2014) Molecular farming of fluorescent virus-based nanoparticles for optical imaging in plants, human cells and mouse models. Biomaterials Sci 2 (5) 784–797.
Shukla S, Wen AM, Ayat NR, Commandeur U, Gopalkrishnan R, Broome AM, Lozada KW, Keri RA, Steinmetz NF (2014) Biodistribution and clearance of a filamentous plant virus in healthy and tumor-bearing mice. Nanomedicine (Lond) 9 (2) 221–235.
Vasilev N, Schmitz C, Dong L, Ritala A, Imseng N, Häkkinen ST, van der Krol S, Eibl R, Oksman-Caldentey K-M, Bouwmeester H, Fischer R, Schillberg S (2014) Comparison of plant-based expression platforms for the heterologous production of geraniol. Plant Cell Tissue Org Cult 117 (3) 373–380.
Vasilev N, Schmitz C, Fischer R, Grömping U, Schillberg S (2014) Assessment of cultivation factors effects that affect biomass and geraniol production in transgenic tobacco cell suspension cultures. PLoS One 9 (8) e104620.
Voepel N, Boes A, Edgue G, Beiss V, Kapelski S, Reimann A, Schillberg S, Pradel G, Fendel R, Scheuermayer M, Spiegel H, Fischer R (2014) Malaria vaccine candidate antigen targeting the pre-erythrocytic stage of Plasmodium falciparum produced at high level in plants. Biotechnol J 9 (11) 1435–1445.
Vogel H, Badapanda C, Knorr E, Vilcinskas A (2014) RNA-sequencing analysis reveals abundant developmental stage-specific and immunity-related genes in the pollen beetle Meligethes aeneus. Insect Mol Biol 23 (1) 98–112.
Wen AM, Steinmetz NF (2014) The aspect ratio of nanoparticle assemblies and the spatial arrangement of ligands can be optimized to enhance the targeting of cancer cells. Adv Healthcare Materials 3 (11) 1739–1744.
Winckler S, Krueger R, Schnitzler T, Zang W, Fischer R, Biselli M (2014) A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach. Bioprocess Biosyst Eng 37 (5) 901–912.
Zhu C, Yang Q, Ni X, Bai C, Sheng Y, Shi L, Capell T, Sandmann G, Christou P (2014) Cloning and functional analysis of the promoters that upregulate carotenogenic gene expression during flower development in Gentiana lutea. Physiol Plant 150 (4) 493–504.
Zielonka S, Ernst AM, Hawat S, Twyman RM, Prüfer D, Noll GA (2014) Characterization of five subgroups of the sieve element occlusion gene family in Glycine max reveals genes encoding non-forisome P-proteins, forisomes and forisome tails. Plant Mol Biol 86 (1–2) 51–67.
Altincicek B, Elashry A, Guz N, Grundler FM, Vilcinskas A, Dehne HW (2013) Next-generation sequencing-based transcriptome analysis of septic-injury responsive genes in the beetle Tribolium castaneum. PLoS One 8 (1) e52004.
Angiari S, Rossi B, Piccio L, Zinselmeyer BH, Budui S, Zenaro E, Della Bianca V, Bach SD, Scarpini E, Bolomini-Vittori M, Piacentino G, Dusi S, Laudanna C, Cross AH, Miller MJ, Constantin G (2013) Regulatory T cells suppress the late phase of the immune response in lymph nodes through P-selectin glycoprotein ligand-1. J Immunol. 191 (11) 5489–5500.
Arcalis E, Stadlmann J, Rademacher T, Marcel S, Sack M, Altmann F, Stoger E (2013) Plant species and organ influence the structure and subcellular localization of recombinant glycoproteins. Plant Mol Biol 83 (1–2) 105–117.
Baptista C, Lopes MS, Tavares AC, Rojer H, Kappmeyer L, Mendonça D, da Câmara Machado A (2013) Diagnosis of Theileria equi infections in horses in the Azores using cELISA and nested PCR. Ticks Tick Borne Dis 4 (3) 242–245.
Bebrone C, Bogaerts P, Delbrück H, Bennink S, Kupper MB, Rezende de Castro R, Glupczynski Y, Hoffmann KM (2013) GES-18, a new carbapenem-hydrolyzing GES-type β lactamase from Pseudomonas aeruginosa that contains Ile80 and Ser170 residues. Antimicrob Agents Chemother 57 (1) 396–401.
Biau A, Santiveri F, Lloveras J (2013) Stover management and nitrogen fertilization effects on corn production. Agron J 105 (5) 1264–1270.
Blazic M, Kovacevic G, Prodanovic O, Ostafe R, Gavrovic-Jankulovic M, Fischer R, Prodanovic R (2013) Yeast surface display for the expression, purification and characterization of wild-type and B11 mutant glucose oxidases. Protein Expr Purif 89 (2) 175–180.
Buyel JF, Fischer R (2013) Processing heterogeneous biomass: overcoming the hurdles in model building. Bioengineered 4 (1) 21–24.
Buyel JF, Kaever T, Buyel JJ, Fischer R (2013) Predictive models for the accumulation of a fluorescent marker protein in tobacco leaves according to the promoter/5'UTR combination. Biotechnol Bioeng 110 (2) 471–482.
Buyel JF, Woo JA, Cramer SM, Fischer R (2013) The use of quantitative structure-activity relationship models to develop optimized processes for the removal of tobacco host cell proteins during biopharmaceutical production. J Chromatogr A 1322, 18–28.
Chen F, Fasoli M, Tornielli GB, Dal Santo S, Pezzotti M, Zhang L, Cai B, Cheng ZM (2013) The evolutionary history and diverse physiological roles of the grapevine calcium-dependent protein kinase gene family. PLoS One 8 (12) e80818.
Dal Santo S, Tornielli G, Zenoni S, Fasoli M, Farina L, Anesi A, Guzzo F, Delledonne M, Pezzotti M (2013) The plasticity of the grapevine berry transcriptome. Genome Biol 14, r54.
Dal Santo S, Vannozzi A, Tornielli GB, Fasoli M, Venturini L, Pezzotti M, Zenoni S (2013) Genome-wide analysis of the expansin gene superfamily reveals grapevine-specific structural and functional characteristics. PLoS One 8 (4) e62206.
Da Silva C, Zamperin G, Ferrarini A, Minio A, Dal Molin A, Venturini L, Buson G, Tononi P, Avanzato C, Zago E, Boido E, Dellacassa E, Gaggero C, Pezzotti M, Carrau F, Delledonne M (2013) The high polyphenol content of grapevine cultivar Tannat berries is conferred primarily by genes that are not shared with the reference genome. Plant Cell 25 (12) 4777–4788.
Daschner de Tercero M, Bruns M, González Martínez I, Türk M, Fehrenbacher U, Jennewein S, Barner L (2013) Continuous hydrothermal synthesis of in situ functionalized iron oxide nanoparticles: A general strategy to produce metal oxide nanoparticles with clickable anchors. Particle Syst Char 30 (3) 229–234.
Dashevskaya S, Horn R, Chudobova I, Schillberg S, Vélez SMV, Capell T, Christou C (2013) Abscisic acid and the herbicide safener cyprosulfamide cooperatively enhance abiotic stress tolerance in rice. Mol Breeding 32 (2) 463–484.
Díaz C, Laurie VF, Molina AM, Bücking M, Fischer R (2013) Characterization of selected organic and mineral components of qvevri wines. Am J Enol Vitic 64, 532–537.
Díaz C, Molina AM, Nähring J, Fischer R (2013) Characterization and dynamic behavior of wild yeast during spontaneous wine fermentation in steel tanks and amphorae. Biomed Res Int 2013, 540465.
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Polesani M, Desario F, Ferrarini A, Zamboni A, Pezzotti M, Kortekamp A, Polverari A (2008) cDNA-AFLP analysis of plant and pathogen genes expressed in grapevine infected with Plasmopara viticola. BMC Genomics 9, 142.
Rademacher T, Sack M, Arcalis E, Stadlmann J, Balzer S, Altmann F, Quendler H, Stiegler G, Kunert R, Fischer R, Stoger E (2008) Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans. Plant Biotechnol J 6 (2) 189–201.
Ramessar K, Rademacher T, Sack M, Stadlmann J, Platis D, Stiegler G, Labrou N, Altmann F, Ma J, Stoger E, Capell T, Christou P (2008) Cost-effective production of a vaginal protein microbicide to prevent HIV transmission. Proc Natl Acad Sci USA 105 (10) 3727–3732.
Schinkel H, Jacobs P, Schillberg S, Wehner M (2008) Infrared picosecond laser for perforation of single plant cells. Biotechnol Bioeng 99 (1) 244–248.
Stahnke B, Thepen T, Stocker M, Rosinke R, Jost E, Fischer R, Tur MK, Barth S (2008) Granzyme B-H22(scFv), a human immunotoxin targeting CD64 in acute myeloid leukemia of monocytic subtypes. Mol Cancer Ther 7 (9) 2924–2932.
Stocker M, Pardo A, Hetzel C, Reutelingsperger C, Fischer R, Barth S (2008) Eukaryotic expression and secretion of EGFP-labeled annexin A5. Protein Expr Purif 58 (2) 325–331.
Truniger V, Nieto C, González-Ibeas D, Aranda MA (2008) Mechanism of plant eIF4E-mediated resistance against a Carmovirus (Tombusviridae): cap-independent translation of a viral RNA controlled in cis by an (a)virulence determinant. Plant J 56 (5) 716–727.
Wullner U, Neef I, Eller A, Kleines M, Tur MK, Barth S (2008) Cell-specific induction of apoptosis by rationally designed bivalent aptamer–siRNA transcripts silencing eukaryotic elongation factor 2. Curr Cancer Drug Targets 8 (7) 554–565.
Zamboni A, Minoia L, Ferrarini A, Tornielli GB, Zago E, Delledonne M, Pezzotti M (2008) Molecular analysis of post-harvest withering in grape by AFLP transcriptional profiling. J Exp Bot 59 (15) 4145–4159.
Zhu C, Naqvi S, Breitenbach J, Sandmann G, Christou P, Capell T (2008) Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize. Proc Natl Acad Sci USA 105 (47) 18232–18237.
Avesani L, Marconi G, Morandini F, Albertini E, Bruschetta M, Bortesi L, Pezzotti M, Porceddu A (2007) Stability of Potato virus X expression vectors is related to insert size: implications for replication models and risk assessment. Transgenic Res 16 (5) 587–597.
Kebeish R, Niessen M, Thiruveedhi K, Bari R, Hirsch HJ, Rosenkranz R, Stäbler N, Schönfeld B, Kreuzaler F, Peterhänsel C (2007) Chloroplastic photorespiratory bypass increases photosynthesis and biomass production in Arabidopsis thaliana. Nature Biotechnol 25 (5) 593–599.
Knoll A, Bartsch S, Husemann B, Engel P, Schroer K, Ribeiro B, Stöckmann C, Seletzky J, Büchs J (2007) High cell density cultivation of recombinant yeasts and bacteria under non-pressurized and pressurized conditions in stirred tank bioreactors. J Biotechnol 132 (2) 167–179.
Mett V, Shamloul AM, Hirai H, Zhou Z, Notkins A, Yusibov V (2007) Engineering and expression of the intracellular domain of insulinoma-associated tyrosine phosphatase (IA-2ic), a type 1 diabetes autoantigen, in plants. Transgenic Res 16 (1) 77–84.
Noll GA, Fontanellaz ME, Rüping B, Ashoub A, van Bel AJ, Fischer R, Knoblauch M, Prüfer D (2007) Spatial and temporal regulation of the forisome gene for1 in the phloem during plant development. Plant Mol Biol 65 (3) 285–294.
Ribbert M, Wolters A, Barth S, Stocker M, Schaeffer A, Fischer R, Finnern R (2007) Immunodetection of Venturia inaequalis ascospores with phage antibodies. J Phytopathol 155 (3) 170–177.
Sack M, Paetz A, Kunert R, Bomble M, Hesse F, Stiegler G, Fischer R, Katinger H, Stoeger E, Rademacher T (2007) Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures. FASEB J 21 (8) 1655–1664.
Schwan S, Fritzsche M, Cismak A, Heilmann A, Spohn U (2007) In vitro investigation of the geometric contraction behavior of chemo-mechanical P-protein aggregates (forisomes). Biophys Chem 125 (2–3) 444–452.
Uhde-Holzem K, Fischer R, Commandeur U (2007) Genetic stability of recombinant Potato virus X virus vectors presenting foreign epitopes. Arch Virol 152 (4) 805–811.
Drakakaki G, Marcel S, Arcalis E, Altmann F, Gonzalez-Melendi P, Fischer R, Christou P, Stoger E (2006) The intracellular fate of a recombinant protein is tissue dependent. Plant Physiol 141 (2) 578–586.
Drakakaki G, Zabotina O, Delgado I, Robert S, Keegstra K, Raikhel N (2006) Arabidopsis reversibly glycosylated polypeptides 1 and 2 are essential for pollen development. Plant Physiol 142 (4) 1480–1492.
Lan P, Li W, Fischer R (2006) Arabidopsis thaliana wild type, pho1, and pho2 mutant plants show different responses to exogenous cytokinins. Plant Physiol Biochem 44 (5–6) 343–350.
Abranches R, Marcel S, Arcalis E, Altmann F, Fevereiro P, Stoger E (2005) Plants as bioreactors: a comparative study suggests that Medicago truncatula is a promising production system. J Biotechnol 120 (1) 121–134.
Agrawal PK, Kohli A, Twyman RM, Christou P (2005) Transformation of plants with multiple cassettes generates simple transgene integration patterns and high expression levels. Mol Breeding 16 (3) 247–260.
Bruell D, Bruns CJ, Yezhelyev M, Huhn M, Müller J, Ischenko I, Fischer R, Finnern R, Jauch KW, Barth S (2005) Recombinant anti-EGFR immunotoxin 425(scFv)-ETA' demonstrates anti-tumor activity against disseminated human pancreatic cancer in nude mice. Int J Mol Med 15 (2) 305–313.
Drakakaki G, Marcel S, Glahn RP, Lund EK, Pariagh S, Fischer R, Christou P, Stoger E (2005) Endosperm-specific co-expression of recombinant soybean ferritin and Aspergillus phytase in maize results in significant increases in the levels of bioavailable iron. Plant Mol Biol 59 (6) 869–880.
Fitter AH, Gilligan CA, Hollingworth K, Kleczkowski A, Twyman RM, Pitchford JW, the members of the NERC Soil Biodiversity Programme (2005) Biodiversity and ecosystem function in soil. Functional Ecol 19 (3) 369–377.
Girardi CL, Corrent AR, Lucchetta L, Zanuzo MR, Zanatta J, Da Costa TS, Twyman RM, Nora FR, Nora L, Silva JA, Rombaldi CV (2005) Effect of ethylene, intermittent warming and controlled atmosphere on postharvest quality and the occurrence of wooliness in peach (Prunus persica cv. Chiripa) during cold storage. Postharvest Biol Technol 38 (1) 25–33.
Kuepper MB, Huhn M, Spiegel H, Ma JK, Barth S, Fischer R, Finnern R (2005) Generation of human antibody fragments against Streptococcus mutans using a phage display chain shuffling approach. BMC Biotechnol 5, 4.
Mehlo L, Gahakwa D, Nghia PT, Loc NT, Capell T, Gatehouse JA, Gatehouse AM, Christou P (2005) An alternative strategy for sustainable pest resistance in genetically enhanced crops. Proc Natl Acad Sci USA 102 (22) 7812–7816.
Nicholson L, Gonzalez-Melendi P, van Dolleweerd C, Tuck H, Perrin Y, Ma JK, Fischer R, Christou P, Stoger E (2005) A recombinant multimeric immunoglobulin expressed in rice shows assembly-dependent subcellular localization in endosperm cells. Plant Biotechnol J 3 (1) 115–127.
Nölke G, Schneider B, Fischer R, Schillberg S (2005) Immunomodulation of polyamine biosynthesis in tobacco plants has a significant impact on polyamine levels and generates a dwarf phenotype. Plant Biotechnol J 3 (2) 237–247.
Paetz A, Sack M, Thepen T, Tur MK, Bruell D, Finnern R, Fischer R, Barth S (2005) Recombinant soluble human Fcγ receptor I with picomolar affinity for immunoglobulin G. Biochem Biophys Res Commun 338 (4) 1811–1817.
Schiermeyer A, Schinkel H, Apel S, Fischer R, Schillberg S (2005) Production of Desmodus rotundus salivary plasminogen activator α1 (DSPAα1) in tobacco is hampered by proteolysis. Biotechnol Bioeng 89 (7) 848–858.
Schinkel H, Schiermeyer A, Soeur R, Fischer R, Schillberg S (2005) Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells. Transgenic Res 14 (3) 251–259.
Stöcker M, Klockenbring T, Huhn M, Nachreiner T, Wicklein D, Petersen A, Bauer R, Goerlich R, Fischer R, Barth S (2005) Antigen-specific targeting and elimination of EBV-transformed B cells by allergen toxins. J Allergy Clin Immunol 116 (4) 910–915.
Uhde K, Fischer R, Commandeur U (2005) Expression of multiple foreign epitopes presented as synthetic antigens on the surface of Potato virus X particles. Arch Virol 150 (2) 327–340.
von der Decken V, Delbrück H, Herrler A, Beier HM, Fischer R, Hoffmann KM (2005) Recombinant bovine uteroglobin at 1.6 Å resolution: a preliminary X-ray crystallographic analysis. Acta Crystallogr Se 61 (5) 499–502.
Arcalis E, Marcel S, Altmann F, Kolarich D, Drakakaki G, Fischer R, Christou P, Stoger E (2004) Unexpected deposition patterns of recombinant proteins in post-endoplasmic reticulum compartments of wheat endosperm. Plant Physiol 136 (3) 3457–3466.
Capell T, Bassie L, Christou P (2004) Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress. Proc Natl Acad Sci USA 101 (26) 9909–9914.
Di Fiore S, Hoppmann V, Fischer R, Schillberg S (2004) Transient gene expression of recombinant terpenoid indole alkaloid enzymes in Catharanthus roseus leaves. Plant Mol Biol Rep 22 (1) 15–22.
Gaur RK, Kupper MB, Fischer R, Hoffmann KMV (2004) Preliminary X-ray analysis of a human VH fragment at 1.8 Å resolution. Acta Cryst D60, 965–967.
Kohli A, Prynne MQ, Miro B, Pereira A,Twyman RM, Capell T, Christou P (2004) Dedifferentiation-mediated changes in transposition behavior make the Activator transposon an ideal tool for functional genomics in rice. Mol Breeding 13 (2) 177–191.
Peschen D, Li HP, Fischer R, Kreuzaler F, Liao YC (2004) Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen. Nature Biotechnol 22 (6) 732–738.
Phlippen N, Hoffmann K, Fischer R, Wolf K, Zimmermann M (2003) The glutathione synthetase of Schizosaccharomyces pombe is synthesized as a homodimer but retains full activity when present as a heterotetramer. J Biol Chem 278 (41) 40152–40161.
Silva JA, da Costa TS, Lucchetta L, Marini LJ, Zanuzo MR, Nora L, Nora FR, Twyman RM, Rombaldi CV (2004) Characterization of ripening behavior in transgenic melons expressing an antisense 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene from apple. Postharvest Biol Technol 32 (3) 263–268.
Sriraman R, Bardor M, Sack M, Vaquero C, Faye L, Fischer R, Finnern R, Lerouge P (2004) Recombinant anti-hCG antibodies retained in the endoplasmic reticulum of transformed plants lack core-xylose and core-α(1,3)-fucose residues. Plant Biotechnol J 2 (4) 279–287.
Vogt U, Pélissier T, Pütz A, Razvi F, Fischer R, Wassenegger M (2004) Viroid-induced RNA silencing of GFP-viroid fusion transgenes does not induce extensive spreading of methylation or transitive silencing. Plant J 38 (1) 107–118.
Li HP, Fu CY, Peschen D, Ling XY, Fischer R, Liao YC (2003) Cloning and characterization of a gene coding for a class I chitin synthase from Fusarium graminearum. Canad J Plant Pathol 25 (3) 240–248.
Trung-Nghia P, Bassie L, Safwat G, Thu-Hang P, Lepri O, Rocha P, Christou P, Capell T (2003) Reduction in the endogenous arginine decarboxylase transcript levels in rice leads to depletion of the putrescine and spermidine pools with no concomitant changes in the expression of downstream genes in the polyamine biosynthetic pathway. Planta 218 (1) 125–134.
Kurek I, Stöger E, Dulberger R, Christou P, Breiman A (2002) Overexpression of the wheat FK506-binding protein 73 (FKBP73) and the heat-induced wheat FKBP77 in transgenic wheat reveals different functions of the two isoforms. Transgenic Res 11 (4) 373–379.
Lepri O, Bassie L, Thu-Hang P, Christou P, Capell T (2002) Endogenous enzyme activities and polyamine levels in diverse rice cultivars depend on the genetic background and are not affected by the presence of the hygromycin phosphotransferase selectable marker. Theor Appl Genet 105 (4) 594–603.
Loc NT, Tinjuangjun P, Gatehouse AMR, Christou P, Gatehouse JA (2002) Linear transgene constructs lacking vector backbone sequences generate transgenic rice plants which accumulate higher levels of proteins conferring insect resistance. Mol Breeding 9 (4) 231–244.
Palacios N, Christou P, Leech M (2002) Regeneration of Lonicera tatarica plants via adventitious organogenesis from cultured stem explants. Plant Cell Rep 20 (9) 808–813.
Thu-Hang P, Bassie L, Safwat G, Trung-Nghia P, Christou P, Capell T (2002) Expression of a heterologous S-adenosylmethionine decarboxylase cDNA in plants demonstrates that changes in S-adenosyl-L-methionine decarboxylase activity determine levels of the higher polyamines spermidine and spermine. Plant Physiol 129 (4) 1744–1754.
Elumalai S, Bassie L, Christou P, Capell T (2001) Development of a novel gene transfer system for Cajanus cajan and expression of a monocot arginine decarboxylase cDNA in transformed cell lines. Plant Physiol Biochem 39 (7–8) 575–582.
Kohli A, Xiong J, Greco R, Christou P, Pereira A (2001) Tagged transcriptome display (TTD) in indica rice using Ac transposition. Mol Genet Genomics 266 (1) 1–11.
Lepri O, Bassie L, Safwat G, Thu-Hang P, Trung-Nghia P, Holtta E, Christou P, Capell T (2001) Over-expression of a cDNA for human ornithine decarboxylase in transgenic rice plants alters the polyamine pool in a tissue-specific manner. Mol Genet Genomics 266 (2) 303–312.
Maqbool SB, Riazuddin S, Loc NT, Gatehouse AMR, Gatehouse JA, Christou P (2001) Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests. Mol Breeding 7 (1) 85–93.
Stöger E, Parker M, Christou P, Casey R (2001) Pea legumin overexpressed in wheat endosperm assembles into an ordered paracrystalline matrix. Plant Physiol 125 (4) 1732–1742.
Tang K, Sun X, Hu Q, Wu A, Lin C-H, Lin H-J, Twyman RM, Christou P, Feng T (2001) Transgenic rice plants expressing the ferredoxin-like protein (AP1) from sweet pepper show enhanced resistance to Xanthomonas oryzae pv. oryzae . Plant Sci 160 (5) 1035–1042.
Torres E, Gonzalez-Melendi P, Stoger E, Shaw P, Twyman RM, Nicholson L, Vaquero C, Fischer R, Christou P, Perrin Y (2001) Native and artificial reticuloplasmins co-accumulate in distinct domains of the endoplasmic reticulum and in post-endoplasmic reticulum compartments. Plant Physiol 127 (3) 1212–1223.
Abranches R, Santos AP, Wegel E, Williams S, Castilho A, Christou P, Shaw P, Stoger E (2000) Widely separated multiple transgene integration sites in wheat chromosomes are brought together at interphase. Plant J 24 (6) 713–723.
Bassie L, Noury M, Lepri O, Lahaye T, Christou P, Capell T (2000) Promoter strength influences polyamine metabolism and morphogenic capacity in transgenic rice tissues expressing the oat adc cDNA constitutively. Transgenic Res 9 (1) 33–42.
Bassie L, Noury M, Wisnieski JP, Topsom L, Christou P, Capell T (2000) Transgenic cell lines as a useful tool to study the biochemistry of down-regulation of an endogenous rice gene using a heterologous diamine oxidase cDNA. Plant Physiol Biochem 38 (10) 729–737.
Drakakaki G, Christou P, Stoger E (2000) Constitutive expression of soybean ferritin cDNA in transgenic wheat and rice results in increased iron levels in vegetative tissues but not in seeds. Transgenic Res 9 (6) 445–452.
Fu X, Duc LT, Fontana S, Bong BB, Tinjuangjun P, Sudhakar D,Twyman RM, Christou P, Kohli A (2000) Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns. Transgenic Res 9 (1) 11–19.
Fu X, Kohli A, Twyman RM, Christou P (2000) Alternative silencing effects involve distinct types of non-spreading cytosine methylation at a three-gene single-copy transgenic locus in rice. Mol Gen Genet 263 (1) 106–118.
GahakwaS D, Maqbool SB, Fu X, Sudhakar D, Christou P, Kohli A (2000) Transgenic rice as a system to study the stability of transgene expression: multiple heterologous transgenes show similar behaviour in diverse genetic backgrounds. Theor Appl Genet 101 (3) 388–399.
Mazithulela G, Sudhakar D, Heckel T, Mehlo L, Christou P, Davies JW, Boulton MI (2000) The Maize streak virus coat protein transcription unit exhibits tissue-specific expression in transgenic rice. Plant Sci 155 (1) 21–29.
Mehlo L, Mazithulela G, Twyman RM, Boulton MI, Davies JW, Christou P (2000) Structural analysis of transgene rearrangements and effects on expression in transgenic maize plants generated by particle bombardment. Maydica 45 (4) 277–287.
Morello L, Frattini M, Giani S, Christou P, Breviario D (2000) Overexpression of the calcium-dependent protein kinase OsCDPK2 in transgenic rice is repressed by light in leaves and disrupts seed development. Transgenic Res 9 (6) 453–462.
Noury M, Bassie L, Lepri O, Kureck I, Christou P, Capell T (2000) A transgenic rice cell lineage expressing the oat arginine decarboxylase (adc) cDNA constitutively accumulates putrescine in callus and seeds but not in vegetative tissues. Plant Mol Biol 43 (4) 537–544.
Perrin Y, Vaquero C, Gerrard I, Sack M, Drossard J, Stoger E, Christou P, Fischer R (2000) Transgenic pea seeds as bioreactors for the production of a single chain Fv fragment (scFv) antibody used for cancer diagnosis and therapy. Mol Breeding 6 (4) 345–352.
Stoger E, Vaquero C, Torres E, Sack M, Nicholson L, Drossard J, Williams S, Keen D, Perrin Y, Christou P, Fischer R (2000) Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies. Plant Mol Biol 42 (4) 583–590.
Tinjuangjun P, Loc, NT, Gatehouse AMR, Gatehouse JA, Christou P (2000) Enhanced insect resistance in Thai rice varieties generated by particle bombardment. Mol Breeding 6 (4) 391–399.
Zeef LA, Christou P, Leech MJ (2000) Transformation of the tropane alkaloid-producing medicinal plant Hyoscyamus muticus by particle bombardment. Transgenic Res 9 (3) 163–168.
Frattini M, Morello L, Breviario D (1999) Rice calcium-dependent protein kinase isoforms OsCDPK2 and OsCDPK11 show different responses to light and different expression patterns during seed development. Plant Mol Biol 41 (6) 753–764.
Hilliou F, Christou P, Leech MJ (1999) Development of an efficient transformation system for Catharanthus roseus cell cultures using particle bombardment. Plant Sci 140 (2) 179–188.
Kohli A, Gahakwa D, Vain P, Laurie DA, Christou P (1999) Transgene expression in rice engineered through particle bombardment: molecular factors controlling stable expression and transgene silencing. Planta 208 (1) 88–97.
Kohli A, Griffiths S, Palacios N, Twyman RM, Vain P, Laurie DA, Christou P (1999) Molecular characterization of transforming plasmid rearrangements in transgenic rice reveals a recombination hotspot in the CaMV 35S promoter and confirms the predominance of microhomology mediated recombination. Plant J 17 (6) 591–601.
Maqbool SB, Christou P (1999) Multiple traits of agronomic importance in transgenic indica rice plants: analysis of transgene integration patterns, expression levels and stability. Mol Breeding 5 (5) 471–480.
Stoger E, Williams S, Keen D, Christou P (1999) Constitutive versus seed specific expression in transgenic wheat: temporal and spatial control. Transgenic Res 8 (2) 73–82.
Stoger E, Williams S, Keen D, Christou P (1999) Molecular characteristics of transgenic wheat and the effect on transgene expression. Transgenic Res 7 (6) 463–471.
Tang K, Tinjuangjun P, Xu Y, Sun X, Gatehouse JA, Ronald PC, Qi H, Lu X, Christou P, Kohli A (1999) Particle bombardment-mediated co-transformation of elite Chinese rice cultivars with genes conferring resistance to bacterial blight and sap-sucking insect pests. Planta 208 (4) 552–563.
Torres E, Vaquero C, Nicholson L, Sack M, Stoger E, Drossard J, Christou P, Fischer R, Perrin Y (1999) Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies. Transgenic Res 8 (6) 441–449.
Vain P, Worland B, Kohli A, Snape JW, Christou P, Allen GC, Thompson WF (1999) Matrix attachment regions increase transgene expression levels and stability in transgenic rice plants and their progeny. Plant J 18 (3) 233–242.
Kohli A, Leech M, Vain P, Laurie DA, Christou P (1998) Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots. Proc Natl Acad Sci USA 95 (12) 7203–7208.
Leech MJ, May K, Hallard D, Verpoorte R, De Luca V, Christou P (1998) Expression of two consecutive genes of a secondary metabolic pathway in transgenic tobacco: molecular diversity influences levels of expression and product accumulation. Plant Mol Biol 38 (5) 765–774.
Maqbool SB, Husnain T, Riazuddin S, Masson L, Christou P (1998) Effective control of yellow stem borer and rice leaf folder in transgenic rice indica varieties Basmati 370 and M 7 using the novel δ-endotoxin cry2A Bacillus thuringiensis gene. Mol Breeding 4 (6) 501–507.
Sudhakar D, Fu X, Stoger E, Williams S, Spence J, Brown DP, Bharathi M, Gatehouse JA, Christou P (1998) Expression and immunolocalisation of the snowdrop lectin, GNA in transgenic rice plants. Transgenic Res 7 (5) 371–378.